ABSTRACT
The purpose of this study was to analyze the inflammatory response of dog's periapical tissues to 17 percent trisodium EDTA salt (pH 8.0) and 1 percent citric acid (pH 2.0). Saline was used as a control. Six adult dogs were used as the biological model of the study. The experimental units comprised 56 roots of mandibular molars (first and second) and premolars (first, second and third). After coronal opening, pulpectomy and root canal instrumentation were performed using the above-mentioned irrigating solutions. After 24 and 48 hours, the animals were euthanized and the teeth and their supporting tissues were removed and histologically processed. The sections were stained with hematoxylin and eosin and analyzed histopathologically with a light microscope at x100 magnification. The histological analysis focused on the occurrence of acute inflammatory response. The presence of swelling, vasodilatation and inflammatory cells were evaluated and the degree of inflammation was determined for each case. Data were analyzed by Fisher's exact test using the SPSS software with a confidence interval of 95 percent (p<0.05). 17 percent EDTA and 1 percent citric acid caused inflammatory responses in dog's periapical tissues with no significant differences to each other or to saline (control) at either the 24-hour (p=0.482) or 48-hour (p=0.377) periods. It may be concluded that the inflammatory response was of mild intensity for the tested substances.
Subject(s)
Animals , Dogs , Biocompatible Materials/therapeutic use , Citric Acid/therapeutic use , Edetic Acid/therapeutic use , Periapical Tissue/drug effects , Root Canal Irrigants/therapeutic use , Bicuspid/pathology , Edema/chemically induced , Edema/pathology , Models, Animal , Molar/pathology , Periapical Periodontitis/chemically induced , Periapical Periodontitis/pathology , Periapical Tissue/pathology , Pulpectomy/methods , Root Canal Preparation/instrumentation , Root Canal Preparation/methods , Time Factors , Vasodilation/drug effectsABSTRACT
O objetivo deste trabalho foi avaliar o efeito antimicrobiano da pasta à base de hidróxido de cálcio empregada como curativo de demora em pré-molares de cäes com lesäo periapical crônica, sendo utilizados 80 canais radiculares. Após comprovaçäo radiográfica da formaçäo de lesöes periapicais induzidas e preparo biomecânico, os canais radiculares foram preenchidos com pasta à base de hidróxido de cálcio associada ao p-monoclorofenol canforado (Calen-PMCC). Decorridos os períodos de 7, 15 e 30 dias, os animais foram sacrificados e os cortes histológicos obtidos corados pelo método de Brown&Bren. O resultado da avaliaçäo histomicrobiológica demonstrou maior efeito antimicrobiano no período de 30 dias. O período de 15 dias apresentou melhores resultados quando comparado ao de sete dias